Canadian Grain Commission
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Prediction of germination energy of malting barley during long-time storage



Recommended practices for testing barley samples for pre-germination using RVA-StarchMaster unit

The following practices are recommended for testing barley samples for pre-germination using RVA-StarchMaster unit:

A – Sample preparation

  1. Clean barley grain using Carter dockage tester. The setting of the kicker is barley No. 6 riddle, No. 6 buckwheat dockage screen at top sieve, solid plain sieve at middle sieve, air control maximum, and speed control # 7. Samples should be stabilized to the room temperature before any measurements and grinding.
  2. Determine the moisture content (%) in samples using NIR.
  3. Mix the cleaned sample thoroughly and take a small representative sample (recommended amount ~300g). This is an important step to ensure that the sample being analyzed represents the whole material. A precision divider or similar device should be used to take an appropriate sub-sample.
  4. Grind the sample using the Falling Number Laboratory Mill 311 (0.8 mm screen). Whole grain samples should be brought to room temperature before grinding. Clean grinder thoroughly after each sample is ground. If not using immediately, store ground sample in cold room.
  5. Thoroughly stir the ground samples before sub-sampling and weighing. Weigh appropriate amount of the ground sample into a canister. Use the moisture content as determined by NIR and the "moisture conversion table" to determine the amount of sample (g) to be weighed into a canister.
  6. Add exactly 25.0 mL of deionized water using an "eppendorf pipette" or a "dispensette pipette".
  7. Mix the content of the canister with a spatula to disperse the sample. Use the paddle to scrape off the sample from a spatula. Insert the paddle into the canister.
Moisture conversion table
M.C. (%) Weight (g) M.C. (%) Weight (g) M.C. (%) Weight (g)
8.0 3.74 9.5 3.80 11.0 3.87
8.1 3.74 9.6 3.81 11.1 3.87
8.2 3.75 9.7 3.81 11.2 3.87
8.3 3.75 9.8 3.81 11.3 3.88
8.4 3.76 9.9 3.82 11.4 3.88
8.5 3.76 10.0 3.82 11.5 3.89
8.6 3.76 10.1 3.83 11.6 3.89
8.7 3.77 10.2 3.83 11.7 3.90
8.8 3.77 10.3 3.84 11.8 3.90
8.9 3.78 10.4 3.84 11.9 3.90
9.0 3.78 10.5 3.84 12.0 3.91
9.1 3.78 10.6 3.85 12.1 3.91
9.2 3.79 10.7 3.85 12.2 3.92
9.3 3.79 10.8 3.86 12.3 3.92
9.4 3.80 10.9 3.86 12.4 3.93
12.5 3.93 14.0 4.00 15.5 4.07
12.6 3.94 14.1 4.00 15.6 4.08
12.7 3.94 14.2 4.01 15.7 4.08
12.8 3.94 14.3 4.01 15.8 4.09
12.9 3.95 14.4 4.02 15.9 4.09
13.0 3.95 14.5 4.02 16.0 4.10
13.1 3.96 14.6 4.03 16.1 4.10
13.2 3.96 14.7 4.03 16.2 4.11
13.3 3.97 14.8 4.04 16.3 4.11
13.4 3.97 14.9 4.04 16.4 4.11
13.5 3.98 15.0 4.05 16.5 4.12
13.6 3.98 15.1 4.05 16.6 4.12
13.7 3.99 15.2 4.06 16.7 4.13
13.8 3.99 15.3 4.06 16.8 4.13
13.9 4.00 15.4 4.07 16.9 4.14

B) Analysis

8. Analyze the samples using the RVA-StarchMaster unit and the program for RVA Stirring Number (SN). This is a standard 3 min program - it heats the sample to 95°C and mixes it for 10 sec at 960 rpm and then at 160 rpm. Record the final viscosity. The results will be displayed in cP (centipoise) units. To convert the numbers to RVA units, divide the cP units by 12.

RVA units = cP ÷ 12

Run the sample in duplicate.

C) Instrument maintenance

9. In order to ensure the accuracy of measurements, you should check the calibration offset daily. Please follow the instructions in your Installation and Operation Manual.

10. In addition, your StarchMaster unit should be calibrated with a standard oil. This should be done once per month. Newport Scientific recommends Cannon Certified Viscosity Standard R47000 (95C). A calibration Checking Kit is available from your Newport Scientific representative. Measure the viscosity of the calibration oil using the CAL95 program (program designed for calibration). The viscosity of the calibration oil should be within ±5% of the recommended value.

For example, if the recommended value is 1223 cP and the reading is between 1162 cP and 1284 cP your unit is ready for analysis. If the reading is outside of this range, your RVA-StarchMaster requires recalibration, please contact your representative. Only the representative can do it for you!